Development Scientist
Oxford Immunotec
Total years of experience :7 years, 5 Months
Sep 2013- Present Oxford Immunotec
Development Scientist Oxford, United Kingdom
- Carrying out Selective T cell and APC isolation from whole blood.
- Immunophenotyping of leukocytes using flow cytometery.
- Carrying out interferon gamma release assays for the detection of latent Tuberculosis and Lyme disease.
- Writing experimental plans and reports for feasibility studies.
- Maintaining stocks of critical reagents through utilisation of a stock management system.
- Presenting experimental data to key scientific staff in monthly meetings.
- Validating Elispot plate reading software.
- Assisting contract engineers with servicing of critical equipment such as automated cell counters and flow cytometers.
Jul 2011- May 2012 Private english language lessons Wicklow, Ireland -April 2013 Private English language teacher
One to One English language lessons to Adults (ages 21 to 46)
- Level testing of new students
- Designing tailored courses for each student
- Preparation of lesson plans using topical subject matter.
- Personalising error correction strategies.
May 2009- Mar 2011 Ludger ltd. Oxford, United Kingdom Glycoprofiling Scientist,
- Purification of N-glycans using Ludger made glycopeptide release kits.
- Exoglycosidase digestion of N-glycans using a range of different enzymes.
- Analysis of released N-glycans, sialic acids and monosaccharides using HPLC.
- Conducting N-glycan structural analysis using mass spectroscopy with the development department.
- Collation, statistical analysis and presentation of N-glycan HPLC data for client reports.
-Creating presentations of sequenced N-glycans to the company using PowerPoint and OHP's.
-Setting up and maintaining a quality management system for the glycoprofiling department of the company.
-Responsible for writing and reviewing new Standard Operating Procedures (SOP's)
Jun 2006- Mar 2009 Bioanalab ltd. Oxford, United Kingdom
Lab Scientist
- Conducting direct and indirect ELISA's of therapeutic monoclonal antibodies.
- Carrying out regulated (GMP, GCLP and GLP) experimental studies.
- Analysing ELISA data using softmax pro reader software.
- Conducting statistical analysis of ELISA data using Microsoft Excel.
- Setting up and maintenance of HPLC instruments.
- Purifying Proteins using RP HPLC and ultrafiltration.
- Preparing reagents such as HPLC buffers, substrate solutions and sample drug dilutions.
- Coupling Biacore chips and conducting binding assays on recombinant monoclonal antibodies using Surface Plasmon Resonance (SPR) (Biacore 3000 instruments)
- Analysing and presenting binding kinetic data for monoclonal antibodies, using GxP validated BIAevaluation software.
-SOP, analytical procedure, batch record and document change control writing and checking.
-Training of new staff in lab conduct and experimental protocols.
-Liaising with company clients.
Aug 2011 The english academy Dublin, Ireland Certificate in English Language Teaching to Adults (CELTA) Cambridge Accredited • Assignments: Focus on the Learner, Focus on Language, Language Skills, Lessons from the Classroom • Organisation of social functions
Sep 2002 Institute of technology tallaght Dublin, Ireland BSc. in Bioanalytical science (Hons.) • First class honors • Science excellence award • Thesis: The effect of lipase production by selected Burkholderia cepacia strains on epithelial cell invasion in Cystic Fibrosis patients.