Consultant Research Scientist
Ocean Life Science Corporation
مجموع سنوات الخبرة :15 years, 5 أشهر
Review and Planning of different research projects of microbiology and molecular biology.
Planning and execution of assigned research project entitled "microbial diversity analysis of extreme/ unique niches of India"
Responcibilitues
Different microbiological techniques are employed for the isolation and characterization of bacteria.
Bacterial strains were identified using Polyphasic approaches including 16S rRNA gene sequencing, morphological analysis, serotyping, carbohydrate typing, biochemical tests, and FAME analysis.
The utilization of food and other industries involves the screening and quantitative analysis of various enzymes, such as amylase, cellulase, chitinase, protease, lipase, and more, which are secreted by microbes.
PCR is commonly employed for gene amplification, including 16S rRNA, acds, iaa genes. Additionally, horizontal GEL-electrophoresis is utilized for analysis of nucleic acids, such as extracted genomic DNA and RNA from microbes and other amplified genes.
Use of molecular cloning techniques to amplify and manipulate many genes including acds and iaaM, subsequently inserting them into vector to facilitate replication and protein expression.
Molecular techniques and SDS-PAGE are employed for the extraction and purification of proteins.
Planning and execution of different research projects of microbiology and molecular biology.
Conduct the laboratory experiments and compiling the research reports for my PhD work entitled "Functional diversity and interactive effect of plant growth promoting rhizobacteria on performance of legumes".
• Different microbiological techniques are employed for the isolation and characterization of bacteria.
• Bacterial strains were identified using Polyphasic approaches including 16S rRNA gene sequencing, morphological analysis, serotyping, carbohydrate typing, biochemical tests.
• Conduct MPN method for testing of coliform bacteria, endotoxin assay to check the contamination level of water.
• Assay of PGP activities in bacterial strains, phosphorous, solubilization, ACC deaminase assay, indole acetic acid (IAA) production, antagonistic activity, siderophore production, exopolysaccharide synthesis, cyanide assay, has been conducted through qualitative and quantitative analysis.
Title of thesis- "Functional diversity and interactive effect of plant growth promoting rhizobacteria on performance of legumes". Planning and execution of experiment of my PhD work "Functional diversity and interactive effect of plant growth promoting rhizobacteria on performance of legumes" Isolation and characterization of bacteria from rhizosphere and other environment DNA extraction and amplication of functional genes of bacteria for identification Screening the Plant growth promoting (PGP) activity of isolated bacteria Antibiotic assay and heavy metal assay Different cereal and horticultural crops grown in greenhouse to check the functional plant growth promoting (PGP) activity of bacteria for preparation of biofertilizer.
Title of Project- "Herbicidal toxicity on plant growth promoting rhizobacteria".
Subsidiary Subjects- Zoology and Chemistry