Specialist lab. technologist
United Genetic Laboratories
Total des années d'expérience :1 years, 11 Mois
Work profile: Neonatal Screening test, LCMS test using UPLC system, VitaminD detection using UPLC and HPLC system, molecular tests including DNA extraction and real-time PCR specially for K-ras mutations HPV and HCV virus Infiction and Factor V mutation.
DNA Extraction from variety of samples, real-time PCR testing for K-ras, N-ras and factor V . Pathgene microarray test for viral infections. Communication with outside laboratories network to process samples for specialized genetic and molecular analysis (unit of sending out samples).
Practical skills
During practical courses I worked on different projects and gained different practical skills such as:
Nucleic acids extraction using kits or manual procedure from variety of samples.
Amplification of Nucleic acids using PCR even convential PCR or real time PCR.
Restriction enzyme digestion using restriction enzymes.
Plasmid cloning.
Detection of nucleic acids by gel electrophoresis.
Preparation different types of media for plant tissue culture and Microbiology.
Plant tissue culture and bacterial culture
Identification of bacteria types using basic methods and test.
Extraction and detection of Proteins.
Excellent skills for using different scientific laboratory instruments such as microscope, microtome, centrifuge, nanodrope, thermal cycler, HPLC system, ULPC system, spectrophotometer, affymetrix microarray unit.
Publication
Wesam Al-Khateeb, Eman Bahar, Jamil Lahham, Dana Schroeder and Emad Hussein. (2013) Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR). Physiology and molecular biology of plants. 19: 157-164.
Awards and Scholarships
Based on merit I hold a scholarship from Yarmouk University for the graduate studies (M. SC degree) which covers mutuition and expenses.
Preparation different types of media (Shoot media, Root media, Callus media) for plant tissue culture supplied with different growth regulators and elements, Seed Sterilization, Inocultion, Measure the growth effects of biotic and abiotic elements, , DNA extraction, PCR and gel electrophoresis.
Outcome: Publish one paper at Physiology and molecular biology of plants journal. with title "Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)."
Excellent Thesis title: “Detection of miRNA species as diagnostic and therapeutic response biomarkers in human colorectal cancer
Excellent Thesis title: “Detection of miRNA species as diagnostic and therapeutic response biomarkers in human colorectal cancer
University: Yarmouk university, Irbid-Jordan.
University: Yarmouk university, Irbid-Jordan.