Assistant Professor
Dolphin PG College of Science & Agriculture, Fatehgarh Sahib
Total des années d'expérience :12 years, 6 Mois
Dolphin PG College is affiliated under Punjabi University, Patiala
Teaching MSc Microbiology students.
Subjects: Bacteriology, Genetic Engineering & Molecular Biology, Recent Advanes in Microbiology, Diagnostics, Techniques in Microbiology.
Supervising Researc Projects of Masters degree students.
Research and Development of Lateral Flow Immunoassays for HIV, Hepatitis, Syphilis, Malaria etc.
Handling all the Plant actiities like Raw material selection, Production, Quality control and assurance.
Development of in Vitro diagnostic test kits for HIV, Hepatitis, Malaria, Dengue etc. based on Antigen-antibodies interaction.
Teaching subjects of MSc Biotechnology and Microbiology
Supervising research Projects
Development of in vitro diagnostic test kits, ELISA
PhD Thesis title: “Cloning, purification and characterization of two putative lipases, Rv1923 (LipD) and Rv2485c (LipQ) from Mycobacterium tuberculosis H37Rv and detection of its probable virulence”. Supervisors: Prof. Jagdeep Kaur and Dr. Anju Srivastava, University of Delhi. Techniques Acquainted with: Immunology ELISA, Development of Rapid diagnostic test kits of HIV, HBsAg, HCV, Malaria, Dengue, Typhoid, hCG etc. Molecular Biology DNA Isolation, Conventional PCR, RT PCR, qPCR, Gene Cloning, Restriction Digestion, Single nucleotide polymorphisms (SNPs), In Situ Hybridization, Electrophoresis, DNA Gel, Gel Doc, Animal Cell Culture. Computer Proficiency Microsoft Word, Microsoft Excel, Microsoft Power Point, EndNote, IBM-SPSS, GraphPad Prism, Gene runner, Densitometry analysis using imageJ software, Comfortable with both Macintosh and Windows operating system. PEER-REVIEW PUBLICATIONS (Total: 10) 1. Gurdyal Singh and Vikas Pahal, 2017. Aptamers: Update to Lateral Flow Immunoassays. Current Trends in Biomedical Engineering and Biosciences, 9(5): DOI: 10.19080/CTBEB.2017.09.555773. 2. Dipendrasinh Jadeja, Nandita Dogra, Stuti Arya, Gurpreet Singh, Gurdyal Singh, Jagdeep Kaur, 2016. Characterization of LipN (Rv2970c) of Mycobacterium tuberculosis H37Rv and its Probable Role in Xenobiotic Degradation. Journal of Cellular Biochemistry, 117(2): 390–401. doi:10.1002/jcb.25285. 3. Prince Manta, Shweta Agarwal, Gurdyal Singh, Satyajit Singh Bhamrah. Formulation, development and sensitivity, specificity comparison of gold, platinum and silver nano particle based HIV ½ and hCG IVD rapid test kit (Immune chromatoghraphic test device). WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES, 2015, Volume 4,, Issue 05,, 1870-1905. 4. Gurdyal Singh, Stuti Arya, Manisha, Jagdeep Kaur, 2014. Rv2485c, a putative lipase of M. tuberculosis: expression purification and biochemical characterization. International Journal of Tropical Disease & Health, Vol. 4(1): 1-17, 2014. 5. Gurdyal Singh, Stuti Arya, Arvind Kumar, Dominic Narang, Jagdeep Kaur, Dec., 2013. Molecular Characterization of Oxidative stress inducible LipD of Mycobacterium tuberculosis H37Rv. Current Microbiology, Vol. 67 (6). DOI 10.1007/s00284-013-0486-3.
Scholarship during Masters from June 2002 to May 2004 from DBT, India. For M.Sc. dissertation, I have worked on “M-Protein profiling of streptococci, RNA typing of Rotaviruses, Western Blotting of M-Protein & Diagnosis of Rotaviruses in feacal samples of equines by RNA Extraction method” during 4th semester of Master degree at National Research Center on Equines, Hisar. In this study we screened five different feacal samples for Rotaviruses by RNA extraction and northern blotting and for streptococci by extraction of M-protein and western blotting. Besides this, I have worked in project on “Micro propagation of sugarcane” during 2th semester of Master degree at Harsac, Ch. Charan Singh Haryana Agriculture University, Hisar.
Overall 2nd position in College