Hanan Saed, كيميائي مختبر

Hanan Saed

كيميائي مختبر

مستشفى حسبو الدولي

Location
Egypt
Education
Bachelor's degree, كيمياء وميكروبيولوجي
Experience
32 years, 9 Months

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Work Experience

Total years of experience :32 years, 9 Months

كيميائي مختبر at مستشفى حسبو الدولي
  • Egypt - Cairo
  • My current job since August 1991

 Hormonal assay
 using ELISA technique for FSH, LH, Prolactine, progesterone, testosterone, Estradiol, T3, T4, TSH & growth hormone, Manually using microreader,
 Semi-automatic “by State Fax& Quantum” and Full- automatic “by IMX”, AXSym and Elecsys 2010.
 Immunology
 (Toxoplasma, Rubella, Cytomegalo virus & Herbs virus). For IgG & IgM
 Hepatitis viruses ( A, B, C) Using ELISA technique
 Semen preparation (swim up technique& density gradient centrifugation) for artificial insemination.
 Semen analysis: manually and Automatic using “Hobson”
 Semen Cryopreservation
 Amino acid detection by thin layer chromatography for serum & Urine

Cytogenetic Lab specialist
 Chromosomal Analysis
 Preparing cultures for :
 - Peripheral blood
 - Amniotic fluid
 - Bone marrow
 - Skin & Other Tissues
 - Fragile-X Syndrome

 Make Harvesting & Slide preparation protocols.

 - Banding techniques:
 (GTG, C, R, QFQ banding & silver staining techniques).

 Karyotyping:
 manually & By Imaging systems
 FISH technique “using Abbott - Vysis Probes”

 - Lymphocytes & Bone Marrow.
 - AneuVysion for prenatal diagnosis.
 - Preimplantation genetic diagnosis on Blastomeres

-DNA Fragmentation test
 Immunology
 (Toxoplasma, Rubella, Cytomegalo virus & Herbs virus). For IgG & IgM
 Hepatitis viruses ( A, B, C) Using ELISA technique
 Biochemistry (Liver Function, Kidney function Lipid profile, Protein “total, albumin, globulin & A/G Ratio”, Heart function, Glucose, fructose amine & glucose tolerance, amylase, and most of blood chemistry using semi-automatic and full automatic analyzer Cobas Integra 400+ & Dimension.
 Hematology
 (Routine blood picture manually & using blood counter OMNI c & Cell Dyne
 Routine blood film & Blood film for Malaria
 Coagulation profile PT, PTT using Stago Coagulation analyser and manually, BT & CT
 ESR
 Hemoglobin variation:
 By ion exchange chromatography using “Hemomate 100”.
 Microbiology
 for bacterial cultures & sensitivity for aerobic & anaerobic bacteria using blood agar, chocolate agar, MacConkey Agar, Cled & Broth media
 Identification for gram negative & gram positive bacteria
 Automatic procedure: using Sensititre (automatic instrument for identification by AP 80 & AP 90 plates and sensitivity by MIC & BP plates).

lab technologist at مستشفى السلام الدولي
  • Kuwait
  • December 2001 to August 2005

 Hormonal assay
 using ELISA technique for FSH, LH, Prolactine, progesterone, testosterone, Estradiol, T3, T4, TSH & growth hormone, Manually using microreader,
 Semi-automatic “by State Fax& Quantum” and Full- automatic “by IMX”, AXSym and Elecsys 2010.
 Immunology
 (Toxoplasma, Rubella, Cytomegalo virus & Herbs virus). For IgG & IgM
 Hepatitis viruses ( A, B, C) Using ELISA technique
 Semen preparation (swim up technique& density gradient centrifugation) for artificial insemination.
 Semen analysis: manually and Automatic using “Hobson”
 Semen Cryopreservation
 Amino acid detection by thin layer chromatography for serum & Urine

Cytogenetic Lab specialist
 Chromosomal Analysis
 Preparing cultures for :
 - Peripheral blood
 - Amniotic fluid
 - Bone marrow
 - Skin & Other Tissues
 - Fragile-X Syndrome

 Make Harvesting & Slide preparation protocols.

 - Banding techniques:
 (GTG, C, R, QFQ banding & silver staining techniques).

 Karyotyping:
 manually & By Imaging systems
 FISH technique “using Abbott - Vysis Probes”

 - Lymphocytes & Bone Marrow.
 - AneuVysion for prenatal diagnosis.
 - Preimplantation genetic diagnosis on Blastomeres

-DNA Fragmentation test
 Immunology
 (Toxoplasma, Rubella, Cytomegalo virus & Herbs virus). For IgG & IgM
 Hepatitis viruses ( A, B, C) Using ELISA technique
 Biochemistry (Liver Function, Kidney function Lipid profile, Protein “total, albumin, globulin & A/G Ratio”, Heart function, Glucose, fructose amine & glucose tolerance, amylase, and most of blood chemistry using semi-automatic and full automatic analyzer Cobas Integra 400+ & Dimension.
 Hematology
 (Routine blood picture manually & using blood counter OMNI c & Cell Dyne
 Routine blood film & Blood film for Malaria
 Coagulation profile PT, PTT using Stago Coagulation analyser and manually, BT & CT
 ESR
 Hemoglobin variation:
 By ion exchange chromatography using “Hemomate 100”.
 Microbiology
 for bacterial cultures & sensitivity for aerobic & anaerobic bacteria using blood agar, chocolate agar, MacConkey Agar, Cled & Broth media
 Identification for gram negative & gram positive bacteria
 Automatic procedure: using Sensititre (automatic instrument for identification by AP 80 & AP 90 plates and sensitivity by MIC & BP plates).

Education

Bachelor's degree, كيمياء وميكروبيولوجي
  • at جامعة عين شمس
  • June 1991

Specialties & Skills

Food Microbiology
Chemical
Microbiology
Environmental Microbiology
Chemistry
computer

Languages

English
Expert