Laborotaries
University of Khartoum
Total des années d'expérience :0 years, 8 Mois
* 26 November 2015 - 12 June 2016
During this Period I work as Researcher in Project which is Detection of Human Papilloma Viruses Type 16 in Sudanese Women Patients with Cervical Carcinoma using Molecular Biology Techniques. I collect 158 of Paraffin Embedded Tissues Blocks from Sudanese Women Patients with Cervical Carcinoma such as malignant or benign from Omdurman Maternity Hospital and National Laboratories of Health. I collect their ages, Clinical information, and degree of cancer differentiation from Hospitals records. I isolate Genomic DNA from Paraffin Embedded Tissues using Guanidine Chloride manual method after sectioning samples using Microtome and de paraffinaization the Blocks using Incubater (heating) and Protienase k. The quality of Genomic DNA measure by using Nano drop. Then, I detect HPV16 in DNA using HPV16 and18 Florescence Diagnostic Kits in some samples to used lately as positive control sample to optimize conventional PCR condition. The positive control samples and remaining sample with different concentration and differents programs of Gradient and Touch down PCR with HPV16 E6 specific primer provide no result in gel electrophoresis at predicted size. So, I use Real time PCR with SYBER GREEN Kit and HPV16 E6 specific primer showing the presence of HPV16 in samples is 10% of Malignant cases and 6% in benign - Lab of Physiology and Molecular Biology+Lab of Leishmania-Department of Zoology, Faculty of Science, University of Khartoum- Department of Virology, Central Lab- Khartoum.
Other Experience:
* 1 -9 November 2016.
Basic course on Systems and Method of University Teaching. Khartoum University Center for Advance Training, University of Khartoum. Sudan.
* 25 -27 October 2016.
Training Course on smart board and Electronic Learning. Melody Press International Foundation In-cooperation with Omdurman Islamic University. Sudan.
* 23-24 October 2016.
Training Course on Understanding Questionnaire Design and SPSS for Data Analysis in Research. 7 VISION Center for Management Training of Human Resource Development, Higher Council for Total Quality and Excellence. Sudan.
* 18 October 2016.
Professional Diploma in the Occupational Safety and Health Administration (OSHA), American Board of Unlimited Thinking.
*24-26 September 2016
Training Period in OSHA (Occupation of Safety and Health Administration). 7 VISION Center for Management Training of Human Resource Development, Higher Council for Total Quality and Excellence. Sudan.
* 5-9 September 2016.
Workshop in Basic of Molecular Biology and Bio bioinformatic. Middle East Organization for Molecular Biology. Sudan.
*11 August - 29 September 2016.
Teacher of English Language. Grade One. Al -Mourtaqa International School. Sudan.
* 8 August 2016.
Licence to Practice Certificate. National Council for Medical and Health Professions. Sudan.
*15 July - 1 August 2012
Training period in the following techniques: Isolation of genomic DNA, Polymerase Chain Reaction and Agarose gel electrophoresis. Department of Molecular Biology, Al_Nee lain Medical Research Center, Faculty of Medicine and Health Science, Al Nee lain University. Sudan.
*1 January - 1 May 2012.
Training Period in Pharmacology and Toxicology Department and Training on various biological studies of medicinal plants as well as phytochemical screening of plants extracts. Medical and Aromatic Plants Research Institute, The National Center for Research, Ministry of Science and Technology. Sudan.
* 16 - 24 October 2011.
Training Period in Department of Molecular Biology, Commission for Biotechnology and Genetic Engineering, National center for Research, Ministry of Science and Technology, Sudan.
2014-2016 Master of Genetic and Molecular Biology. University of Khartoum. Sudan. *2013 Qualifying Year. Institute of Endemic Disease. University of Khartoum. Sudan. *2008-2012 Bachelor of Science in Biotechnology. Faculty of Science and Technology. Omdurman Islamic University. Sudan. Diploma: *5 May -5 August 2012 Diploma in Molecular Biology. Golden Care Center for Language, Management Training of Human Resource Development. Khartoum_Sudan. *March- 2011-June 2012 Diploma in Advance English Communication Course. Kaplan Institute for Science and Computer Technology. Sudan. *20 November 2010 -21March 2011 Diploma in English Communication. Al-danga Academy for Computer Sciences and Languages. Sudan. *5 February 2011 - 5 May 2011 Diploma in Computer Application + Printing Skills. M. E. C. for Computer Science. Sudan. Two dissertation: 1- The Effect of Plants Extracts on Diabetic Rats. 2- Detection of HPV16 in Sudanese Women Patients with Cervical Carcinoma.