Researcher
University of Turku
Total years of experience :9 years, 1 Months
Panning and screening of antibody library by phage display technology.
Designing and development (cloning, expression, production and purification) of protein/antibody.
Characterization of antibody (affinity, specificity).
In vitro diagnostic products (IVD's) tests.
Development of a highly sensitive sandwich type TRF immunoassay using monoclonal or Fab antibodies.
Optimization and validation process of the immunoassays measuring the biomarkers of prostate cancer
Problem solving, equipment and process optimization
Developing qualification procedures
Maintenance of the documents of the reagent stock and working on calibration.
Collection, processing and storing of blood and urine samples from hospitals.
Writing scientific article for publication
•Instrumentation:
Cell Culture, Cell Count, Paper Chromatography, Electrophoresis (Agarose Gel Electrophoresis), SDS-PAGE (Sodium dodesyl sulphate polyacrylamide gel electrophoresis), Western bloting, ELISA (Antibody and Cytokine), Cell proliferation study, Chromosome Count, Expression determination of different gene, Immunocyto (histo) chemistry, RT-PCR, VICTOR TM (1420 Multi label counter), UV-Visible and Nanodrop Spectrophotometer, Microplate reader, Microplate washer, Sorvall Ultracentrifuge, HPLC, Sonicator, Bio-Rad Experion (protein affinity measurement) etc.
Panning and screening of antibody library by phage display technology.
Cloning, expression, production and purification of protein/antibody.
Characterization of antibody (affinity, specificity).
Development of a highly sensitive sandwich type TRF immunoassay using monoclonal or Fab antibodies.
Performed different immunological, biochemical and serological tests related to infectious diseases and hormones etc. as my job responsibilities. I also maintained documents of reagents stock, Quality control, and Calibration. I have been versatile in different types of automated analysers such as Vitors 250, Humaster 600 etc. For the coagulation profile, I have been competent of ACL 2000 automated coagulation analyser and adapted in special tests such as hormone analysis by Chemiluminesence in-vitros ECI automated analyser and ELISA. I have handled the electrolyte analyzers, Starlyte Tm, EasyLytePlus etc. I was also expert in troubleshooting and possessed problem solving skills. I also have experience in training newly recruited Biochemist.
Performed different immunological, biochemical and serological tests related to infectious diseases (HIV/AIDS, Hepatitis panel, Malaria, Tuberculosis (TB), Tropical diseases) and hormones.
Master Thesis topic: Panning and Screening of Streptavidin specific antibodies with phage display technology. Thesis work was carried out in the Department of Biotechnology, University of Turku, Finland. Project: Quantitative geno-and chemotyping of cyanotoxin-producing organisms and their toxins in water and solid food matrices.
Subjects studied: Protein Engineering and Molecular Biology, Environmental & Food Technology, Advanced cell Biology, Oncology, Immunology and Advanced Metabolism.
Subjects studied: Basic Biochemistry, Clinical Biochemistry, Pharmacology, Microbiology, Molecular Biology, Human Nutrition, Physiology, Biotechnique, Organic, Inorganic & Physical Chemistry.