Assistant Professor/Molecular Microbiologist
Jawaharlal Nehru Cancer Hospital and Research Centre
Total des années d'expérience :17 years, 10 Mois
Working in Department of Research as Molecular Microbiologist and Assistant Professor in Paramedical Sciences, Jawaharlal Nehru Cancer Hospital and Research Centre, Bhopal
Working in Department of Research as Microbiologist and Assistant Professor in Paramedical Sciences, Jawaharlal Nehru Cancer Hospital and Research Centre, Bhopal
Work responsibilities include handling of the techniques related to tissue Culture and maintenance of different Mammalian cell lines and taking classes of students of Molecular biology, animal tissue culture and Microbiology.
Having research experience in the field of Animal Tissue Culture of 5 years
TECHNICAL SKILLS KNOWN
Cell Culture: Maintenance of Mammalian cell lines from ATCC and NCCS. B16F10 (Mouse Skin Melanoma), B16F0 (Mouse Skin Melanoma), B16F0 (Mouse Skin Melanoma), NIH3T3 (Mouse Fibroblast), MRC-5 & WI-38 (Human Lung Fibroblast), Jurkat (Human Lymphoblastic), B/CMBA.OV (Mouse Ovary), MM55K (Mouse Kidney), NCTC- CLONE (Mouse Liver), HCN-1A (Human Cortical Neuron), HPAE-26 (Human Pulmonary Artery), HFAE-2 (Human Femoral Artery), GC-1spg (Mouse testis; transformed with pSV3-neo), IMR-90 (Human Lung), Hela (Human Cervix), T-24 (Human Bladder, transitional cell carcinoma), HT-29 (Human Colorectal; Adenocarcinoma), ZR-75-I (Human Mammary gland; Ductal carcinoma), MCF-7 (Human Mammary gland; Breast; Adenocarcinoma), 293T/17 (Human Kidney; transformed with adenovirus 5DNA), FCH (Human Colon)
Growth in Agar, - Galactosidase staining.
Molecular Biology: DNA Extraction, Gel Electrophoresis, PCR, Cloning, Plasmid Extraction PCR Purification and Restriction Enzyme Digestion.
Immunology: ELISA
HANDLING OF INSTRUMENTS
Having technical experience in handling following instruments
• Phase Contrast (Z focus) Microscope with Digital Camera
• ACT - 1 Software. Nikon
• Inverted Phase Contrast Microscope
•Ultracentrifuge & High speed centrifuge
•PCR
• Electrophoresis Instruments
•Epi-Fluorescence Microscope
•Gel Documentation
Advance Laboratory Techniques and Research Protocol from Jawaharlal Nehru Cancer Hospital and Research Centre, Bhopal
Work experience : Take classes of High School and High Secondary students
Diagnosis of Aujeszky’s Disease (Psuedorabies) Virus Infection in Porcine Species.
Institution: High Security Animal Disease Laboratory
Grade First Class
Microbiology Years Attended
Years Attended
Grade First Class
/ Madhmik Shiksha Mandal,