Mohammad Saquib Ashraf, Medical Laboratory Scientist/Assistant Professor

Mohammad Saquib Ashraf

Medical Laboratory Scientist/Assistant Professor

Shaqra University

البلد
الهند - Darbhanga
التعليم
دكتوراة, Genomics Hematology
الخبرات
11 years, 3 أشهر

مشاركة سيرتي الذاتية

حظر المستخدم


الخبرة العملية

مجموع سنوات الخبرة :11 years, 3 أشهر

Medical Laboratory Scientist/Assistant Professor في Shaqra University
  • المملكة العربية السعودية - الرياض
  • نوفمبر 2018 إلى أغسطس 2020

• Worked on research projects as PI, Co-I, Co-PI.

• Conducted PCR, DNA sequencing, and cell culture bio-assay regularly.

• Utilized expertise in microbiology, biochemistry, and hematology to help with the diagnosis of medical issues.

• Handled specimens according to laboratory protocols to maximize accuracy and precision of results.

• Wrote research papers, reports, reviews, and summaries regarding molecular biology, cell biology, clinical laboratory science.

• Provided qualified input and development support for student plans and study protocols carried out by students.

Clinical Laboratory Scientist/Assistant Professor في University Of Hail
  • المملكة العربية السعودية - حائل
  • فبراير 2015 إلى أغسطس 2018

• Developed direct research activities independently including data collection, evaluation and analyses for a research. Assisted the Principal Investigator in the preparation of manuscripts and grant applications in my college.

• Prepared course syllabi and collaborated in writing modules for Dynamic Learning System

• Worked on research projects as PI, Co-I, Co-PI.

• Led a research team of more than two dozen of students on graduation projects and successfully met all key milestones.

• Utilized expertise in microbiology, biochemistry, and hematology to help with the diagnosis of medical issues.

• Handled specimens according to laboratory protocols to maximize accuracy and precision of results.

• Wrote research papers, reports, reviews, and summaries regarding molecular biology, cell biology, clinical laboratory science.

• Provided qualified input and development support for student plans and study protocols carried out by students.

• Conducted studies on bio-active molecules and metabolites and the role of each in drug development.

• Developed case report forms and conducted medical monitoring in partnership with hospitals and medical personnel.

Doctoral Fellow في University of Rome Tor Vergata
  • إيطاليا
  • أكتوبر 2010 إلى يونيو 2014

• As a doctoral fellow I had been involved in the development of biomarkers and novel targeted therapies for acute myeloid leukemia. Where my gene of interest is IDH2 (R172K) and I tried to understand the role of Oncoprotein and a transcription factor involved in the signaling pathway of IDH.

• I had been involved in the novel detection method of newly discovered NADP-dependent mitochondrial IDH1 and IDH2 mutations in Acute myeloid leukemia patients through Sanger Sequencing and ASO-PCR- an easy, economic, and less time consuming technique.

• Got expertise in molecular and cellular bio-assay's techniques including cloning, cell culture, evaluation of protein stability and expression in-vitro, following treatment with available effective chemotherapeutic drugs used in leukemias.

• Have been involved in the immunological analysis through flow-cytometry, cell cycle, apoptosis, and cell differentiation in-vitro and in patient’s sample. I extensively worked on human bone marrow cells and immortalized cell lines.

• Maintain and characterize cancer cell lines viz NB4, U937, HL-60

• Perform cell treatment following existing SOPs. Cell culture: adherent and suspension, use automation to increase throughput of cell-based assays, Flow-cytometry (FACS), MTT Assay, cytotoxicity, Immunofluorescence, mycoplasma detection.

• Perform cellular mechanism of action studies

• Researching, developing and applying systems to develop molecular & cell biology-based assays

• All elements of experiments, including planning, execution, quantitative data analysis, and interpretation, are carried out with the intent of enhancing assay-related protocols and data analysis methodologies.

• Generated modified cell lines using transfection or viral transduction, then use flow cytometry to analyze cellular function.

• Designed and constructed by specific antibody screening assays using functional cell-based assays.

Senior Researcher في All India Institute Of Medical Sciences
  • الهند - دلهي
  • فبراير 2009 إلى أغسطس 2010

Study of P38 map kinase in Oral cancer patients

Research Scholar في All India Institute Of Medical Sciences
  • الهند - New Delhi
  • أغسطس 2008 إلى فبراير 2009

Master Degree project dissertation

الخلفية التعليمية

دكتوراة, Genomics Hematology
  • في University Of Rome Tor Vergata
  • يونيو 2014
ماجستير, Biotechnology
  • في Bundelkhand University
  • فبراير 2009
بكالوريوس, B-Tech(Biotechnology)
  • في Bundelkhand University, Jhansi
  • مايو 2007

Production of Biodiesel from Jatropha plant

Specialties & Skills

Clinical Research
Genomics
Cell Biology
Molecular Biology
DNA sequencing
Teaching
Researcher
Oncologist
Hematologist
Molecular Biologist
Cell Culture
DNA Sequencing
Microbiology
Biochemistry
teamwork
research
laboratory medicine
medical devices
phlebotomy
university teaching
laboratory analysis
lab testing

حسابات مواقع التواصل الاجتماعي

الموقع الشخصي
الموقع الشخصي

لقد تم حذف الرابط بسبب انتهاكه لسياسة الموقع. يرجى التواصل مع قسم الدعم لمزيد من المعلومات.

اللغات

الانجليزية
متمرّس
الهندية
اللغة الأم
الأوردو
اللغة الأم

العضويات

EACR (European Association of Cancer research),
  • Active member
  • January 2011
ESH (European School of Hematology).
  • Active member
  • February 2012
EHA (European Hematology Association),
  • Active member
  • January 2012
MEMBS (Middle East Molecular Biology Sources)
  • Active member
  • January 2016

التدريب و الشهادات

Dissertation (تدريب)
معهد التدريب:
All India Institute of Medical Sciences
تاريخ الدورة:
August 2008
المدة:
4320 ساعة

الهوايات

  • Cricket
  • Singing
  • Writting