محمد أرشد, Research Assistant

محمد أرشد

Research Assistant

King Saud University

البلد
المملكة العربية السعودية - الرياض
التعليم
ماجستير, Microbiology
الخبرات
5 years, 7 أشهر

مشاركة سيرتي الذاتية

حظر المستخدم


الخبرة العملية

مجموع سنوات الخبرة :5 years, 7 أشهر

Research Assistant في King Saud University
  • المملكة العربية السعودية - الرياض
  • مارس 2015 إلى أبريل 2015

 Optimization and expression of proteins in recombinant E.coli stains.
 Bacterial Transformation
 Purification of Protein (Native and Denaturing Methods, Ni-NTA Spin Kit)
 Affinity Chromatography, Ion exchange chromatography.
 SDS-PAGE and Western Blotting (Bio-Rad, Gene script).
 Dialysis of proteins
 Protein estimation using Bradford assay.
 RNA extraction from nasopharyngeal Aspirates from Children of KSA.
 Real time PCR of extracted RNA from Nasopharyngeal aspirates
 cDNA synthesis optimization (Applied Biosystem, Invitrogen)
 Sequencing PCR of positive RSV samples

Trainee في King Khaled Eye Specialist Hospital
  • المملكة العربية السعودية - الرياض
  • ديسمبر 2014 إلى فبراير 2015

 Sample Collection (Iris, Cornea, Aqueous Humour, Donor Rims) from Operation Room/Clinical Labs.
 Sample processing for RNA Extraction by Snap Freezing
 DNA Extraction from Blood samples (QIAGEN, Gentra puregene blood kit)
 RNA Extraction from Corneal tissues (Optimization, RNeasy mini kit)
 Nanodrop (Thermoscientific 2000/2000c)
 Primer designing
 Gel Electrophoresis
 Polymerase Chain Reaction (Gradient PCR, Hot Star PCR) and its Optimization (Bio- rad)
 Purification of PCR product (Column Clean-up)
 Target/Sanger Gene Sequencing (Applied Bio systems Hitachi 3730 DNA analyzer)
 Sequence Analysis (DNASTAR Laser gene Software, Bioinformatics tools)
 Monitoring cell culture room (maintaining Stocks, monitoring CO2 incubators, Inverted Microscopes).
 Dissection of corneal rims by Dissection Microscope (Olympus TH4-200)
 Monitoring freezers and Refrigerators (4 oC, -20 oC, -80 oC)
 Periodic Maintenance of Liquid Nitrogen Levels.
 Weekly cleaning of sequencer
 Maintaining records of blood, tissue samples from patients
 Racking of tips, autoclaving liquids, instruments and lab wares.
 Detecting Antigens (Proteins) in tissue samples using Immunohistochemistry technique (Autostainer link DACO)

Researcher في King Saud University
  • المملكة العربية السعودية - الرياض
  • سبتمبر 2009 إلى أكتوبر 2014

• Samples preparation of biologically active molecules such as indole & omeprazole derivatives, pyrazoles, thiazoles, pyrimidines, etc., for Nuclear Magnetic Resonance
• Worked on Autochem analyzer to ascertain Temperature Programmed Reduction and Oxidation values of polymer samples.
• Ensuring complete in-process quality control and continuous improvement in process capabilities.
• Ordering, monitoring and maintaining records pertaining to chemicals and disposables in the lab.
• Technical presentations of samples processed at periodic intervals and deliver training to new graduate students

الخلفية التعليمية

ماجستير, Microbiology
  • في King Saud University
  • يونيو 2014

Thesis Entitled "Polyhydroxy butyrate (PHB) production by Bacillus sp. isolated from Mangrove environments of eastern coast of Saudi Arabia".

بكالوريوس, Biotechnology
  • في Bhavans New Science College
  • يونيو 2008

Worked On various Molecular biology and Medical microbiology Techniques.

Specialties & Skills

Western Blotting
DNA sequencing
Agarose Gel Electrophoresis
Bacterial Culture

اللغات

العربية
متوسط
الانجليزية
متمرّس
الأوردو
متمرّس

الهوايات

  • Reading Research articles, Music.