Research Assistant
King Saud University
Total years of experience :5 years, 7 Months
Optimization and expression of proteins in recombinant E.coli stains.
Bacterial Transformation
Purification of Protein (Native and Denaturing Methods, Ni-NTA Spin Kit)
Affinity Chromatography, Ion exchange chromatography.
SDS-PAGE and Western Blotting (Bio-Rad, Gene script).
Dialysis of proteins
Protein estimation using Bradford assay.
RNA extraction from nasopharyngeal Aspirates from Children of KSA.
Real time PCR of extracted RNA from Nasopharyngeal aspirates
cDNA synthesis optimization (Applied Biosystem, Invitrogen)
Sequencing PCR of positive RSV samples
Sample Collection (Iris, Cornea, Aqueous Humour, Donor Rims) from Operation Room/Clinical Labs.
Sample processing for RNA Extraction by Snap Freezing
DNA Extraction from Blood samples (QIAGEN, Gentra puregene blood kit)
RNA Extraction from Corneal tissues (Optimization, RNeasy mini kit)
Nanodrop (Thermoscientific 2000/2000c)
Primer designing
Gel Electrophoresis
Polymerase Chain Reaction (Gradient PCR, Hot Star PCR) and its Optimization (Bio- rad)
Purification of PCR product (Column Clean-up)
Target/Sanger Gene Sequencing (Applied Bio systems Hitachi 3730 DNA analyzer)
Sequence Analysis (DNASTAR Laser gene Software, Bioinformatics tools)
Monitoring cell culture room (maintaining Stocks, monitoring CO2 incubators, Inverted Microscopes).
Dissection of corneal rims by Dissection Microscope (Olympus TH4-200)
Monitoring freezers and Refrigerators (4 oC, -20 oC, -80 oC)
Periodic Maintenance of Liquid Nitrogen Levels.
Weekly cleaning of sequencer
Maintaining records of blood, tissue samples from patients
Racking of tips, autoclaving liquids, instruments and lab wares.
Detecting Antigens (Proteins) in tissue samples using Immunohistochemistry technique (Autostainer link DACO)
• Samples preparation of biologically active molecules such as indole & omeprazole derivatives, pyrazoles, thiazoles, pyrimidines, etc., for Nuclear Magnetic Resonance
• Worked on Autochem analyzer to ascertain Temperature Programmed Reduction and Oxidation values of polymer samples.
• Ensuring complete in-process quality control and continuous improvement in process capabilities.
• Ordering, monitoring and maintaining records pertaining to chemicals and disposables in the lab.
• Technical presentations of samples processed at periodic intervals and deliver training to new graduate students
Thesis Entitled "Polyhydroxy butyrate (PHB) production by Bacillus sp. isolated from Mangrove environments of eastern coast of Saudi Arabia".
Worked On various Molecular biology and Medical microbiology Techniques.