Research Professor
Wonkwang University
Total years of experience :12 years, 10 Months
Antibody Engineering, Molecular Cloning, Phage display, Protein-Protein Interaction, Expression and purification of Proteins
The microbial risk assessment for “Fish safety and Quality Assurance” was done by using microbial methods, GC, HPLC, LC-MS and ICP-MS and “Application of Bioactive peptide derived from Marine Fish”.
“Potential application of antibody-mimicking peptides identified by phage display from monoclonal and polyclonal antibodies raised in rabbit” and “Development of phage display and antibody engineering technology for the production of antibody fragments suitable for detecting agricultural contaminants”, Phage Display Technology and Antibody Engineering
Targeted drug delivery using peptide probes and Myocardial sensing peptides using Phage Display Technology
Optimization and Production of enzymes from laboratory to Industrial scale.
Cell death is a fundamental biological process that is present in numerous disease pathologies. Preclinically, chemotherapeutic efficacies are monitored by various in vitro apoptotic tests. Real time, non invasive in vivo imaging and quantification of therapy-induced tumor apoptosis would impact drug discovery significantly. Here we describe a near infrared (NIR) conjugated peptide, named ApoPep-2 (Apoptosis-targeting Peptide-2), for in vivo imaging of cell death. ApoPep-2 also bound to apoptotic cells in culture, while only little binding to live cells was observed. Fluorescence microscopy showed the number of fluorescent Apopep-2 bound cells was significantly increased after etoposide treatment. Chemically induced in vivo models of Apoptosis were established using Doxorubicin via tail vein injection in nude mice implanted with A549 lung tumor cells. The fluorescent Apopep-2 probe or corresponding control was subsequently injected and whole animal fluorescence imaging demonstrated probe uptake at the site of apoptosis, which was confirmed by ex vivo and histological analyses. Further, a comparative study with a near-infrared fluorescence labeled Apopep-2 and Annexin V showed high intense uptake at the site of tumor. The results indicate that ApoPep-2 is an effective probe for in vivo cell death detection and in some cases may be an appropriate alternative to Annexin V conjugates for the detection, quantification and monitoring of apoptosis following cancer treatments.
“Development of a Cost Effective Medium for Protease production using Bacillus sp.” Under the guidance of Dr. M.K Gowthaman, Scientist EII, Central Leather Research Institute, Adyar, Chennai-600 020 The enzyme protease was produced and optimized in flask level and scaled it up to 3L, 20L and 300L fermentors using Hi-media components. For economic feasibility, the production cost has to be reduced. In this study, different commercially available carbon and nitrogen sources are tried out for the protease production. Finally, a suitable source has been found and has been scaled it up to 3L fermentor. The cost has been reduced by 10 fold and the production has been increased twice when compared to medium with Hi-media components. Instruments used: • Basic microbiology instruments. • Fermentors. • Continuous centrifuge. • Ultra filtration unit
“Transformation of Green Fluorescent Protein into Tobacco plant using Agro bacterium mediated transformation” in Sreedhar Bhatt’s laboratory Bangalore. Plasmid DNA containing Annexin gene was selected. Using restriction digestion, Annexin gene was isolated. Then the gene was transferred into Agrobacterium via Tri-parental mating (TPM). Followed by transformation of Annexin gene from Bacterial system to Plant system. Transformation was confirmed by Polymerase Chain Reaction (PCR).