Yusuf Maaeni,  Office of Agriculture Research /Poultry Research Station /Abu Ghraib /Iraq

Yusuf Maaeni

Office of Agriculture Research /Poultry Research Station /Abu Ghraib /Iraq

Ministry of Agriculture

Location
Iraq
Education
Doctorate, V. good
Experience
8 years, 4 Months

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Work Experience

Total years of experience :8 years, 4 Months

Office of Agriculture Research /Poultry Research Station /Abu Ghraib /Iraq at Ministry of Agriculture
  • Iraq - Baghdad
  • August 2008 to November 2016

Researcher in the field of animal production / genetic engineering. I work in the poultry breeding and a improving this strains by using genetic engineering techniques and gene transfer. Moreover, I implemented the practical most important part of my doctoral thesis under name (CLONING AND IDENTIFICATION OF JAPANESE QUAIL (COTURNIX COTURNIX JAPONICA) FOLLISTATIN GENE) in the biologica laboratory to Dr. Kim at the University of Hawaii at Manoa, USA.

Education

Doctorate, V. good
  • at College of Agriculture, University of Baghdad
  • June 2015

Specialties & Skills

Protein Purification
Tissue Culture
SDS PAGE
Protein Expression
RNA isolation
expression vector cloning (Gibson Assembly® Cloning Kit), expression induction,validation of protein
expression vector selection (pMAL™ Protein Fusion & Purification System)
A high capability to interacting well with other lab members
Purification of recombinant proteins using affinity chromatography (amylose, heparin resin columns
cDNA synthesis using First Choice® RLM-RACE Kit, cDNA cloning techniques for sequence analysis,
SDS-PAGE techniques used for protein identification
Estimation of protein concentration (UV method and Lowry method)
Extraction of RNA from tissue,E. coli culture techniques and transformation of E. coli and plasmid
Examination of myostatin-inhibitory activity using HEK293 (CAGA) 12 Luciferase Assay system.

Languages

English
Intermediate

Training and Certifications

Laboratory and Biological satety, Environmental Health, Blood borne pathogens (Training)
Training Institute:
Certificate of training / University ofHawaii at Manoa
Date Attended:
November 2013
Duration:
50 hours