principle investigator
university of the Punjab Lahore
مجموع سنوات الخبرة :1 years, 3 أشهر
Isolation and Characterization of Lytic Bacteriophages against Escherichia coli.
In an era where antibiotic resistance is causing many problems particularly in nosocomial situations, phage and phage-based technologies may prove to be valuable antimicrobial alternatives for widespread applications in the future. The antibiotic resistance, clinical need and the availability of suitable bacteriophages combine to make this a potential ‘perfect storm’ with which to progress this new (but also very old) therapeutic approach into the clinic. Here, we aimed to isolate and further characterize suitable bacteriophages which may be useful in fighting back the hospital acquired infections. This may suggest as a valuable addition in the phage therapy research work to be applied clinically.
Honours Degree Microbiology And Molecular Genetics from the University of the Punjab, Qaida -e-Azam Campus Lahore, graduated in 20013. Thesis topic: "Isolation and Characterization of Bacteriophages Against Multi- drug resistance Escherichia coli" Lab techniques skills * Can efficiently perform PCR tests, having Experience of Nested PCR, Reverse Transcriptase PCR and Amplification PCR. * Having skills in Gel Electrophoresis. * DNA and RNA isolation. * ELISA * Transformation * Sterility Test for Raw Materials, in process and Finished Products * Sub culturing of Micro-organism to be performed as per SOP. Instrument Handled PCR Thermocycler, Optical Density metre, Gel Doc. Machine, Autoclave, Vortex machine, Electromagnetic stirrer, Sonicator, Centrifuge machine, Incubator, Gel Electrophoresis Apparatus, Laminar Air Flow, UV- illuminator, Colony Counter, Microscope, pH Meter, Incubator, Autoclave.