Dr Habib ur Rehman

Practical approach (performed with my own hands): Serological tests : 1) Haemagglutination test (HA); 2) Haemagglutination inhibition test (HI); 3) Indirect Haemagglutination test (IHA); 4) Enzyme linked immunosorbant assay (ELISA); 5) Compliment Fixation Test (CFT); 6) Agar gel precipitation test (AGPT); 7) Counter current immuno-electrophoresis etc Cell culture technique: produce multiple cells (cell line) from a single cell Invitro AND then infect with pure virus to see the cytopathic effects of virus on cells. Bacterial identification tests: catalase test; Starch hydrolysis test; (Performed repeatedly Mannitol fermentation; Citrate test; On Glucose fermentation; Oxidase test; H2S production; Indole production; Methyl red (MR); VP test; Urease test; Vaccine production: • Vaccine against Newcastle disease virus; • Bivalent vaccine by combining both, virus and bacteria i.e. 3 strains of FMD virus and Pasturella multocida. • Bulk growth of pasturella multocida alone, using biomass fermentor for vaccine production. • growing of virus in fertilized egg. • preparation of hyper immune serum after multiple injections of antigen. Molecular techniques: • SDS-PAGE (sodium Dodecyl Sulphate,Polyacrilamide Gel Electrophoresis) • DNA extraction • Plasmid extraction • Competent cell preparation Workshops:  Preparation and evaluation of oil based Foot and Mouth Disease (FMD) and Hemorrhagic Septicemia Vaccine.  Propagation of Newcastle Disease Virus in chicken embryos and detection of its immunologic protein using SDS-PAGE.  Bio-mass production of Pasturella Multocida for vaccine preparation  Modern techniques in angiogenesis and toxicology Certificates : Certificate of participation in 6th ASIAN BUFFALO CONGRESS Certificate as member of hospitality committee in 6th ASIAN BUFFALO CONGREE.

To diagnose,treat and eradicate animal diseases. Management practices 4 months internship in dairy sector ( HALEEB FOODS ) Winner of university merit scholarship during DVM.